Letizia Barbieri was born in 1985. She graduated in Chemistry in 2010 at the University of Florence under the guidance of professor Ivano Bertini. She obtained the International PhD in “Mechanistic and Structural Systems Biology” under the guidance of professor Lucia Banci in 2014 at the University of Florence. Currently she is Research Assistant at the Interuniversity Consortium for Magnetic Resonance of Metallo Proteins (CIRMMP, University of Florence, Italy).
Letizia Barbieri is skilled in “in-cell NMR”. She has developed a protocol to achieve high expression levels of proteins in human cells HEK293T by transient DNA transfection in order to characterize biological molecules, their interactions and their functional processes in their native environment, at atomic resolution. Recently, she is involved in the development of NMR bioreactors to allow longer NMR experiments. She is also skilled in preparing proteins to be studied by NMR-based structural biology and by protein-drug interactions.
She has currently published 17 papers in peer-reviewed international journals.
In-cell NMR; Structural Biology; Protein-Protein interaction; Drug screening; NMR bioreactor.
1) Luchinat E., Barbieri L., Campbell T.F., Banci L., Real-Time Quantitative In-Cell NMR: Ligand Binding and Protein Oxidation Monitored in Human Cells Using Multivariate Curve Resolution, Anal. Chem. 2020, 92, 9997−10006
2) Luchinat E., Barbieri L., Cremonini M., Nocentini A., Supuran C.T., Banci L., Drug screening in human cells by NMR allows early assessment of drug potency, Angew. Chem. Int. Ed. 2020, 59, 6535 –6539 doi.org/10.1002/anie.201913436
3) Cerofolini L., Giuntini S., Barbieri L., Pennestri M., Codina A., Fragai M., Banci L., Luchinat E., Ravera E., Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions. Biophys J. 2019, 116, 239-247.
4) Capper, M. J.; Wright, G. S. A.; Barbieri, L.; Luchinat, E.; Mercatelli, E.; McAlary, L.; Yerbury, J. J.; O’Neill, P. M.; Antonyuk, S. V.; Banci, L., Hasnain, S. S., The Cysteine-Reactive Small Molecule Ebselen Facilitates Effective SOD1 Maturation. Nat Commun, 9, 1693 (2018)
5) Barbieri, L., Luchinat, E., Banci, L., Intracellular metal binding and redox behavior of human DJ1. J. Biol. Inorg. Chem, 23, 61-69 (2018)
6) Luchinat, E., Barbieri, L., Banci, L., A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants, Scientific Reports 2017 7(1):17433
7) Barbieri, L., Luchinat, E., Banci, L., Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells, Nature Protocols 11: 1101-1111, 2016
8) Mercatelli, E., Barbieri, L., Luchinat, E., Banci, L., Direct Structural Eevidence of Protein Redox Regulation Obtained by in-cell NMR, BBA - Molecular Cell Research, 1863: 198-204, 2016
9) Barbieri L., Luchinat E., Banci L., Protein interaction patterns in different cellular environments are revealed by in cell NMR. Scientific Reports 5:14456: DOI: 10.1038/srep14456, 2015
10) Luchinat, E., Barbieri, L., Rubino, J. T., Kozyreva, T., Cantini, F., Banci, L., In living cells folding and metal binding of SOD1 fALS mutants is impaired, Nat.Commun., 5, 5502-, 2014
11) Banci, L., Barbieri, L., Bertini, I., Luchinat, E., Secci, E., Zhao, Y., Aricescu, A. R., Atomic-resolution monitoring of protein maturation in live human cells by NMR, Nat.Chem.Biol., 9, 297-299, 2013