AFFILIATION:
Magnetic Resonance Center and Department of Chemistry
University of Florence

ADDRESS:
Via L. Sacconi 6
50019 Sesto Fiorentino, (FI) -Italy

CONTACTS:
Tel: +39 055 4574240
e-mail: parigi[at]cerm.unifi.it ; giacomo.parigi[at]unifi.it

RESEARCH PROFILES:
ORCID_ID profile
Google scholar profile
University of Florence page
Educational

Giacomo Parigi is Associate Professor of Chemistry at the University of Florence.
He was born in 1967, degree in Physics cum laude at the University of Florence (1992) and PhD in Chemistry at the University of Florence. Postdoctor, Researcher (1999-2006) and associate professor of Chemistry (2006-) at CERM, University of Florence.
His research activity is mainly oriented to the analysis of paramagnetic effects in proteins and complexes with paramagnetic metal ions, in the frame of the nuclear magnetic resonance (NMR) studies for the structural and dynamic characterization of biological molecules. These researches are aimed at the definition of improved methodologies for an accurate determination of the structures of proteins and protein complexes in solution, and of their mobility, through NMR spectroscopy. Another field of interest is water proton relaxivity in the presence of paramagnetic complexes, like contrast agents for magnetic resonance imaging (MRI) or paramagnetic molecules for dynamic nuclear polarization (DNP), as well as of protein protons, for getting information on protein mobility and aggregation.
He is co-author with I. Bertini and C. Luchinat of the book "Solution NMR of Paramagnetic Molecules" (Elsevier, 2001), and with I. Bertini, C. Luchinat and E. Ravera of the book "NMR of Paramagnetic Molecules" (Elsevier, 2017). He is co-editor with I. Bertini and K.S. McGreevy of the book "NMR of Biomolecules: Towards Mechanistic Systems Biology" (Wiley-VCH, 2012) and with C. Luchinat and E. Ravera of the book "Paramagnetism in Experimental Biomolecular NMR" (RSC, 2018).

Full CV

RESEARCH FIELDS:

nuclear and electron relaxation, paramagnetism-based effects in NMR, MRI contrast agents, FFC-relaxometry, protein structure and dynamics, bioinformatics.

SELECTED PUBLICATIONS:

J.-H. Tang, H. Li, C. Yuan, G. Parigi, C. Luchinat, T.J. Meade, “Molecular Engineering of Self-Immolative Bioresponsive MR Imaging Probes”, J. Am. Chem. Soc. (2023) 145, 10045–10050

F. Carniato, M. Ricci, L. Tei, F. Garello, C. Furlan, E. Terreno, E. Ravera, G. Parigi, C. Luchinat, M. Botta, “Novel nanogels loaded with Mn(II) chelates as effective and biologically stable MRI probes”, Small (2023) 19, 2302868

L. Cerofolini, K. Vasa, E. Bianconi, M. Salobehaj, G. Cappelli, A. Bonciani, G. Licciardi, A. Pérez-Ràfols, L. Padilla-Cortés, S. Antonacci, D. Rizzo, E. Ravera, C. Viglianisi, V. Calderone, G. Parigi, C. Luchinat, A. Macchiarulo, S. Menichetti, M. Fragai, “Combining Solid-State NMR with Structural and Biophysical Techniques to Design Challenging Protein-Drug Conjugates”, Angew. Chem. Int. Ed. (2023) 62, e2023032

E. Ravera, L. Gigli, E.A. Suturina, V. Calderone, M. Fragai, G. Parigi, C. Luchinat, “A high-resolution view of the coordination environment in a paramagnetic metalloprotein from its magnetic properties” Angew. Chem. Int. Ed. (2021) 60, 14960-14966

Z. Wang, S. Pisano, V. Ghini, P. Kadeřávek, M. Zachrdla, P. Pelupessy, M. Kazmierczak, T. Marquardsen, J.-M. Tyburn, G. Bouvignies, G. Parigi, C. Luchinat, F. Ferrage, “Detection of metabolite-protein interactions in complex biological samples by high-resolution relaxometry: towards interactomics by NMR”, J. Am. Chem. Soc. (2021) 143, 9393–9404

G. Parigi, E. Ravera, M. Fragai, C. Luchinat, “Unveiling protein dynamics in solution with field-cycling NMR relaxometry”, Prog. NMR Spectrosc. (2021), 124–125, 85–98

G. Parigi, E. Ravera, C. Luchinat, “Magnetic susceptibility and paramagnetism-based NMR” Prog. NMR Spectrosc. (2019) 114-115, 211-236.

H. Li, G. Parigi, C. Luchinat, T.J. Meade, “Bimodal Fluorescence-Magnetic Resonance Contrast Agent for Apoptosis Imaging”, J. Am. Chem. Soc. (2019) 141, 6224-6233.

N. Rezaei-Ghaleh, G. Parigi, A. Soranno, A. Holla, S. Becker, B. Schuler, C. Luchinat, M. Zweckstetter, “Local and Global Dynamics in Intrinsically Disordered Synuclein”, Angew.Chem.Int.Ed. (2018) 57, 15262-15266.

G. Liu, M. Levien, N. Karschin, G. Parigi, C. Luchinat, M. Bennati, “One-thousand fold enhancement of high field liquid nuclear-magnetic resonance signals at room temperature”, Nat. Chem. (2017) 9, 676-680.

W. Andrałojć, Y. Hiruma, W.-M. Liu, E. Ravera, M. Nojiri, G. Parigi, C. Luchinat, M. Ubbink, “Identification of productive and futile encounters in an electron transfer protein complex”, Proc. Natl. Acad. Sci. USA (2017) 114, E1840-E1847.

L. Benda, J. Mareš, E. Ravera, G. Parigi, C. Luchinat, M. Kaupp, J. Vaara, “Pseudo-contact NMR shifts over a paramagnetic metalloprotein (CoMMP-12) from first principles”, Angew.Chem.Int.Ed. (2016) 55, 14713-14717.

A. Carlon, E. Ravera, J. Hennig, G. Parigi, M. Sattler, C. Luchinat, “Improved accuracy from joint X-ray and NMR refinement of a protein-RNA complex structure”, J. Am. Chem. Soc. (2016) 138, 1601-1610.

M. Rotz, K. Culver, G. Parigi, K. MacRenaris, C. Luchinat, T. Odom, T.J. Meade, “High Relaxivity Gd(III)-DNA Gold Nanostars: Investigation of Shape Effects on Proton Relaxation”, ACS Nano (2015) 9, 3385-3396

Complete list of publications